产品详情
简单介绍：
重组牛干扰素τ蛋白与其它公司提供的重组蛋白不同，rOvIFN-τ蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组牛干扰素τ蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组牛干扰素τ蛋白
| Species | Ovine |
| Accession | P56828 |
| GeneID | 100144750 |
| Source | Yeast |
| Molecular Weight | 重组牛干扰素τ蛋白Approximately 19.9 kDa, a single glycosylated polypeptide chain containing 172 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ EMVEGDQLQK DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT LLEQLCTGLQ QQLDHLDTCR GQVMGEEDSE LGNMDPIVTV KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR ALTVSTTLQK RLTKMGGDLN SP |
| Purity | 重组牛干扰素τ蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral resistance assay is no less than 1.0 × 107IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重组牛干扰素τ蛋白Less than 0.1 EU/µg of rOvIFN-τ as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组牛干扰素τ蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Clayette P, Martin M, Dereuddre-Bosquet N, et al. 1999. Pathol Biol (Paris), 47: 553-9. 2. Tekin S, Ealy AD, Wang SZ, et al. 2000. J Interferon Cytokine Res, 20: 1001-5. 3. Tennakoon DK, Smith R, Stewart MD, et al. 2001. J Interferon Cytokine Res, 21: 785-92. 4. Ezashi TandRoberts RM. 2004. Endocrinology, 145: 4452-60. 5. Asselin E, Lacroix D, Fortier MA. 1997. Mol Cell Endocrinol, 132: 117-26. |
| Background | IFN-τ is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN-τ has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-α/β. The current investigation concerns the effect of recombinant ovine IFN-tau (rOvIFN-τ) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and up regulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS is via interference with IFN-γ-induced MHC class II expression. IFN-τwas shown to down regulate IFN-γ-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in down regulating inflammation in the central nervous system (CNS). IFN-τdid not upregulate the expression of MHC class II on CVE. IFN-τalso inhibited the replication of Theiler's virus in CVE. |
