产品详情
简单介绍：
重组猪白细胞介素-2蛋白与其它公司提供的重组蛋白不同，rPoIL-2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组猪白细胞介素-2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组猪白细胞介素-2蛋白
| Synonyms | T-cell Growth Factor, TCGF |
| Species | Porcine |
| Accession | P26891 |
| GeneID | 396868 |
| Source | 重组猪白细胞介素-2蛋白Escherichia coli. |
| Molecular Weight | Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT |
| Purity | 重组猪白细胞介素-2蛋白> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4. |
| Endotoxin | 重组猪白细胞介素-2蛋白Less than 1 EU/μg of rPoIL-2 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组猪白细胞介素-2蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79. 2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10. 3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51. 4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25. 5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6. |
| Background | IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant porcine IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 72 % amino acid sequence identity with mouse, human and rat IL-2. It also shares 60 % and 67 % acid sequence identity with rhesus macaque and equus caballus IL-2, respectively. |
