产品详情
简单介绍：
重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白与其它公司提供的重组蛋白不同，rRtLIX/CXCL5蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白
| Synonyms | Cytokine LIX, Small-inducible Cytokine B5 |
| Species | Rat |
| Accession | P97885 |
| GeneID | 60665 |
| Source | 重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白Escherichia coli. |
| Molecular Weight | Approximately 10.1 kDa, a single, non-glycosylated polypeptide chain containing 93 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtLIX/CXCL5 as determined by LAL method. |
| Reconstitution | 重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠LPS诱导的CXC趋化因子/ CXCL5蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7. |
| Background | CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-γ. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57 % amino acid sequence identity with human CXCL5. |
