产品详情
简单介绍：
重组大鼠胶质细胞成熟因子beta蛋白与其它公司提供的重组蛋白不同，rRtGMF-β蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠胶质细胞成熟因子beta蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠胶质细胞成熟因子beta蛋白
| Synonyms | GMFB |
| Species | Rat |
| Accession | Q63228 |
| GeneID | 81661 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠胶质细胞成熟因子beta蛋白Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | SESLVVCDVA EDLVEKLRKF RFRKETHNAA IIMKIDKDKR LVVLDEELEG VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPLGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | 重组大鼠胶质细胞成熟因子beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtGMF-β as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重组大鼠胶质细胞成熟因子beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠胶质细胞成熟因子beta蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7. 2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8. 3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25. 4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82. |
| Background | The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells. |
