产品详情
简单介绍：
重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白与其它公司提供的重组蛋白不同，rRtIL-1RA蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白
| Synonyms | IL-1RN, IRAP, IL1 Inhibitor |
| Species | Rat |
| Accession | P25086 |
| GeneID | 60582 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Quantity | 20µg/100µg/1000µg |
| AA Sequence | HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白Fully biologically active when compared to standard. The ED50as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103IU/mg in the presence of 50 pg/ml rRtIL-1α. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rRtIL-1RA as determined by LAL method. |
| Reconstitution | 重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠白细胞介素-1受体拮抗剂蛋白蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Dinarello CA. 1994. FASEB J. 8:1314-25. 2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6. 3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7. 4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94. 5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13. |
| Background | IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89% and 73% a.a. sequence homology with murine and human IL-1RA. |
