产品详情
简单介绍：
重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白与其它公司提供的重组蛋白不同，rRtTNF-α/TNFSF2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白
| Synonyms | Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin |
| Species | Rat |
| Accession | P16599 |
| GeneID | 24835 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白Fully biologically active when compared to standard. The ED50as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rRtTNF-α as determined by LAL method. |
| Reconstitution | 重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠肿瘤坏死因子-α/ TNFSF2蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9. 2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483. 3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41. 4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202. |
| Background | Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
