产品详情
简单介绍：
重组大鼠白细胞介素-21蛋白与其它公司提供的重组蛋白不同，rRtIL-21蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠白细胞介素-21蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠白细胞介素-21蛋白
| Synonyms | Za11 |
| Species | Rat |
| Accession | A3QPB9 |
| GeneID | 365769 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠白细胞介素-21蛋白Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠白细胞介素-21蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human N1186 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtIL-21 as determined by LAL method. |
| Reconstitution | 重组大鼠白细胞介素-21蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠白细胞介素-21蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43. 2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91. 3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264. 4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32. |
| Background | Rat IL-21 is produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, it promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. Mature rat IL-21 shares 88% a.a. sequence identity with mouse IL-21. |
