产品详情
简单介绍：
重组大鼠白细胞介素-17A蛋白与其它公司提供的重组蛋白不同，rRtIL-17A蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠白细胞介素-17A蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠白细胞介素-17A蛋白
| Species | Rat |
| Accession | Q61453 |
| Unigene | Rn.218513. |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠白细胞介素-17A蛋白Approximately 30.0 kDa, a disulfide-linked homodimer of two 133 amino acid polypeptide chains. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | AVLIPQSSVC PNAEANNFLQ NVKVNLKVIN SLSSKASSRR PSDYLNRSTS PWTLSRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPEKCPFT FRVEKMLVGV GCTCVSSIVR HAS |
| Purity | 重组大鼠白细胞介素-17A蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重组大鼠白细胞介素-17A蛋白Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 6.5. |
| Endotoxin | Less than 0.1 EU/µg of rRtIL-17A as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠白细胞介素-17A蛋白 |
