产品详情
简单介绍：
重组鼠单核细胞趋化蛋白-3 / CCL7蛋白与其它公司提供的重组蛋白不同，rMuMCP-3/CCL7蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠单核细胞趋化蛋白-3 / CCL7蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠单核细胞趋化蛋白-3 / CCL7蛋白
| Synonyms | Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC |
| Species | Murine |
| Accession | Q03366 |
| GeneID | 20306 |
| Source | Escherichia coli. |
| Molecular Weight | 重组鼠单核细胞趋化蛋白-3 / CCL7蛋白Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组鼠单核细胞趋化蛋白-3 / CCL7蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 2× PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rMuMCP-3/CCL7 as determined by LAL method. |
| Reconstitution | 重组鼠单核细胞趋化蛋白-3 / CCL7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组鼠单核细胞趋化蛋白-3 / CCL7蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8. 2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9. |
| Background | Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic function for monocytes and eosinophils, but not for neutrophils. |
