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  • 产品名称：重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白

  • 产品型号：rMuDHH C23II
  • 产品厂商：KALANG
  • 产品文档：
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简单介绍：
重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白与其它公司提供的重组蛋白不同，rMuDHH C23II蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：

重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白

Species Murine
Accession Q61488
GeneID 13363
Source Escherichia coli.
Molecular Weight 重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.
Quantity 5µg/25µg/1000µg
AA Sequence IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
Purity > 98 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20 μg/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
Endotoxin Less than 0.1 EU/μg of rMuDHH C23II as determined by LAL method.
Reconstitution 重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠沙漠Hedgehog同系物Cys23IleIle蛋白
SDS-PAGE

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