产品详情
简单介绍：
重组鼠粘膜相关上皮趋化因子/ CCL28蛋白与其它公司提供的重组蛋白不同，rMuMEC/CCL28蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠粘膜相关上皮趋化因子/ CCL28蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠粘膜相关上皮趋化因子/ CCL28蛋白
| Synonyms | CCK1 |
| Species | Murine |
| Accession | Q9JIL2 |
| GeneID | 56838 |
| Source | Escherichia coli. |
| Molecular Weight | 重组鼠粘膜相关上皮趋化因子/ CCL28蛋白Approximately 12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组鼠粘膜相关上皮趋化因子/ CCL28蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rMuMEC/CCL28 as determined by LAL method. |
| Reconstitution | 重组鼠粘膜相关上皮趋化因子/ CCL28蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组鼠粘膜相关上皮趋化因子/ CCL28蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Wang W, Soto H, Oldham ER, et al. 2000. J Biol Chem, 275: 22313-23. 2. Hieshima K, Ohtani H, Shibano M, et al. 2003. J Immunol, 170: 1452-61. 3. Eksteen B, Miles A, Curbishley SM, et al. 2006. J Immunol, 177: 593-603. 4. Kagami S, Kakinuma T, Saeki H, et al. 2005. J Invest Dermatol, 124: 1088-90. |
| Background | CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. |
