产品详情
简单介绍：
重组鼠肝素结合EGF样生长因子蛋白与其它公司提供的重组蛋白不同，rMuHB-EGF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠肝素结合EGF样生长因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠肝素结合EGF样生长因子蛋白
| Synonyms | HBEGF, DT-R |
| Species | Murine |
| Accession | Q06186 |
| GeneID | 15200 |
| Source | Escherichia coli. |
| Molecular Weight | 重组鼠肝素结合EGF样生长因子蛋白Approximately 9.8 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
| Quantity | 10µg/50µg/1000µg |
| AA Sequence | DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组鼠肝素结合EGF样生长因子蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH7.4. |
| Endotoxin | 重组鼠肝素结合EGF样生长因子蛋白Less than 1 EU/μg of rMuHB-EGF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| 重组鼠肝素结合EGF样生长因子蛋白 | |
| Reference | 1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73. 2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9. |
| Background | Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. The protein is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Mouse HB-EGF shares about 73 % a.a. sequence identity with human HB-EGF. |
