产品详情
简单介绍：
重组小鼠胰岛素样生长因子-1蛋白与其它公司提供的重组蛋白不同，rMuIGF-1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠胰岛素样生长因子-1蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组小鼠胰岛素样生长因子-1蛋白
| Species | Murine |
| Accession | P05017 |
| GeneID | 16000 |
| Source | Escherichia coli. |
| Molecular Weight | 重组小鼠胰岛素样生长因子-1蛋白Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 10µg/50µg/1000µg |
| AA Sequence | GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组小鼠胰岛素样生长因子-1蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | 重组小鼠胰岛素样生长因子-1蛋白Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组小鼠胰岛素样生长因子-1蛋白 |
