产品详情
简单介绍：
干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白与其它公司提供的重组蛋白不同，rMuMIG/CXCL9蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白
| Synonyms | Gamma-interferon-induced Monokine, Small-inducible Cytokine B9 |
| Species | Murine |
| Accession | P18340 |
| GeneID | 17329 |
| Source | 干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白Escherichia coli. |
| Molecular Weight | Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 105 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rMuMIG/CXCL9 as determined by LAL method. |
| Reconstitution | 干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22. 4. Adamska I, Roobol-Boza M, Lindahl M, et al. 1999. Eur J Biochem. 260:453-60. |
| Background | CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-γ. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9. |
