产品详情
简单介绍：
重组鼠巨噬细胞集落刺激因子蛋白与其它公司提供的重组蛋白不同，rMuM-CSF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠巨噬细胞集落刺激因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠巨噬细胞集落刺激因子蛋白
| Synonyms | CSF-1, MGI-IM |
| Species | Murine |
| Accession | Q3U4F9 |
| Unigene | Mm.795. |
| Source | Escherichia coli. |
| Molecular Weight | 重组鼠巨噬细胞集落刺激因子蛋白Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
| Purity | 重组鼠巨噬细胞集落刺激因子蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
| Endotoxin | 重组鼠巨噬细胞集落刺激因子蛋白Less than 1 EU/μg of rMuM-CSF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组鼠巨噬细胞集落刺激因子蛋白 | |
| Reference | 1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26. 2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52. 3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35. 4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102. |
| Background | Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229 a.a. of mature mouse M-CSF shares 87 %, 83 %, 82 % and 81 % sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
