产品详情
简单介绍：
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白与其它公司提供的重组蛋白不同，rMuEotaxin/CCL11蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白
| Synonyms | Small-inducible Cytokine A11 |
| Species | Murine |
| Accession | P48298 |
| GeneID | 20292 |
| Source | Escherichia coli. |
| Molecular Weight | 重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method. |
| Reconstitution | 重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12. 2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7. 3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56. 4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5. |
| Background | Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor. |
