产品详情
简单介绍：
重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白与其它公司提供的重组蛋白不同，rMuMIP-1γ/CCL9/CCL10蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白
| Synonyms | MIP-1-gamma, MRP-2, Small-inducible Cytokine A9 |
| Species | Murine |
| Accession | P51670 |
| GeneID | 20308 |
| Source | 重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白Escherichia coli. |
| Molecular Weight | Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method. |
| Reconstitution | 重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Youn BS, Jang IK, Broxmeyer HE, et al. 1995. J Immunol. 155:2661-7. 2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51. 3. Lean JM, Murphy C, Fuller K, et al. 2002. J Cell Biochem. 87:386-93. |
| Background | Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10. |
