产品详情
简单介绍:
重组人**细胞激活基因1蛋白/ CCL4L1蛋白与其它公司提供的重组蛋白不同,rHuLAG-1/CCL4L1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人**细胞激活基因1蛋白/ CCL4L1蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍:
重组人**细胞激活基因1蛋白/ CCL4L1蛋白
Species | Human |
Accession | Q8NHW4 |
GeneID | 388372 |
Source | Escherichia coli. |
Molecular Weight | 重组人**细胞激活基因1蛋白/ CCL4L1蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
Quantity | 5µg/20µg/1000µg |
AA Sequence | APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity | 重组人**细胞激活基因1蛋白/ CCL4L1蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
Endotoxin | 重组人**细胞激活基因1蛋白/ CCL4L1蛋白Less than 0.1 EU/μg of rHuLAG-1/CCL4L1 as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions with a NaCl concentration no less than 300 mM. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles. |
重组人**细胞激活基因1蛋白/ CCL4L1蛋白 | |
SDS-PAGE |