产品详情
简单介绍：
重组人类印度刺猬Cys28IleIle蛋白与其它公司提供的重组蛋白不同，rHuIHH C28II蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类印度刺猬Cys28IleIle蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类印度刺猬Cys28IleIle蛋白
| Synonyms | HHG-2 |
| Species | Human |
| Accession | Q14623 |
| GeneID | 3549 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类印度刺猬Cys28IleIle蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类印度刺猬Cys28IleIle蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rHuIHH C28II as determined by LAL method. |
| Reconstitution | 重组人类印度刺猬Cys28IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类印度刺猬Cys28IleIle蛋白 | |
| Reference | 1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72. 2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51. 3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22. |
| Background | IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in ***** kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with murine. |
