产品详情
简单介绍：
重组人成纤维细胞生长因子4蛋白与其它公司提供的重组蛋白不同，rHuFGF-4蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人成纤维细胞生长因子4蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人成纤维细胞生长因子4蛋白
| Synonyms | HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3 |
| Species | Human |
| Accession | P08620 |
| GeneID | 2249 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人成纤维细胞生长因子4蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
| Purity | 重组人成纤维细胞生长因子4蛋白> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl. |
| Endotoxin | 重组人成纤维细胞生长因子4蛋白Less than 1 EU/µg of rHuFGF-4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人成纤维细胞生长因子4蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6. 2. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 3. Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45. |
| Background | FGF-4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family. |
