产品详情
简单介绍：
重组人黑色素瘤抑制剂活性蛋白2蛋白与其它公司提供的重组蛋白不同，rHuMIA-2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人黑色素瘤抑制剂活性蛋白2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人黑色素瘤抑制剂活性蛋白2蛋白
| Species | Human |
| Accession | Q96PC5 |
| GeneID | 117153 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 11.4 kDa, a single non-glycosylated polypeptide chain containing 100 amino acids. |
| Quantity | 重组人黑色素瘤抑制剂活性蛋白2蛋白5µg/20µg/1000µg |
| AA Sequence | LESTKLLADL KKCGDLECEA LINRVSAMRD YRGPDCRYLN FTKGEEISVY VKLAGEREDL WAGSKGKEFG YFPRDAVQIE EVFISEEIQM STKESDFLCL |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | 重组人黑色素瘤抑制剂活性蛋白2蛋白Less than 1 EU/µg of rHuMIA2 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重组人黑色素瘤抑制剂活性蛋白2蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人黑色素瘤抑制剂活性蛋白2蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Hellerbrand C, Amann T, Schlegel J, et al. 2008. Gut, 57: 243-51. 2. Bosserhoff AK, Moser M, Scholmerich J, et al. 2003. J Biol Chem, 278: 15225-31. 3. Pitman JL, Bonnet DJ, Curtiss LK, et al. 2011. J Lipid Res, 52: 1775-86. |
| Background | Melanoma Inhibitor Activity Protein 2 (MIA-2) encoded by MIA2 gene in humans, is a secreted cytokine that is highly expressed in liver and weakly in testis. The patients with severe fibrosis or inflammation and chronic hepatitis have higher level of MIA2 than other. Levels of MIA2 may severe as a clinically utility marker for diagnosis of hepatic disease activity and severity. The MIA2 is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain. |
