产品详情
简单介绍：
重组人泛素偶联酶E2 L3，His蛋白与其它公司提供的重组蛋白不同，rHuUBE2L3, His蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人泛素偶联酶E2 L3，His蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人泛素偶联酶E2 L3，His蛋白
| Synonyms | L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3 |
| Species | Human |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| Quantity | 10µg/50µg/1000µg |
| AA Sequence | 重组人泛素偶联酶E2 L3，His蛋白MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD |
| Concentration | See label. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | 重组人泛素偶联酶E2 L3，His蛋白Sterile Colorless liquid. |
| Formulation | A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
| Endotoxin | Less than 1 EU/µg of rHuUBE2L3, His as determined by LAL method. |
| Storage | Store at -20 °C. |
| 重组人泛素偶联酶E2 L3，His蛋白 | |
| Reference | 1. Serniwka SAandShaw GS. 2008. Biomol NMR Assign, 2: 21-3. 2. Wenzel DM, Lissounov A, Brzovic PS, et al. 2011. Nature, 474: 105-8. 3. Whitcomb EAandTaylor A. 2009. Cell Div, 4: 17. 4. Moynihan TP, Ardley HC, Nuber U, et al. 1999. J Biol Chem, 274: 30963-8. |
| Background | 重组人泛素偶联酶E2 L3，His蛋白Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-proteinshown to interact with UBOX5, ARIH1, Cbl gene, UBE3A and NEDD4. Down-regulated during the S-phase it is involved in progression through the cell cycle. Additionally, UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis. |
