产品详情
简单介绍：
重组人Neuroserpin蛋白与其它公司提供的重组蛋白不同，rHuNeuroserpin蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人Neuroserpin蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人Neuroserpin蛋白
| Synonyms | Peptidase Inhibitor 12, PI-12, Serpin I1, NSP |
| Species | Human |
| Accession | Q99574 |
| GeneID | 5274 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人Neuroserpin蛋白Approximately 44.7 kDa, a single non-glycosylated polypeptide chain containing 394 amino acid residues. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | 重组人Neuroserpin蛋白TGATFPEEAI ADLSVNMYNR LRATGEDENI LFSPLSIALA MGMMELGAQG STQKEIRHSM GYDSLKNGEE FSFLKEFSNM VTAKESQYVM KIANSLFVQN GFHVNEEFLQ MMKKYFNAAV NHVDFSQNVA VANYINKWVE NNTNNLVKDL VSPRDFDAAT YLALINAVYF KGNWKSQFRP ENTRTFSFTK DDESEVQIPM MYQQGEFYYG EFSDGSNEAG GIYQVLEIPY EGDEISMMLV LSRQEVPLAT LEPLVKAQLV EEWANSVKKQ KVEVYLPRFT VEQEIDLKDV LKALGITEIF IKDANLTGLS DNKEIFLSKA IHKSFLEVNE EGSEAAAVSG MIAISRMAVL YPQVIVDHPF FFLIRNRRTG TILFMGRVMH PETMNTSGHD FEEL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人Neuroserpin蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using rat C6 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuNeuroserpin as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人Neuroserpin蛋白 | |
| Reference | 1. Berger P, Kozlov SV, Cinelli P, et al. 1999. Mol Cell Neurosci, 14: 455-67. 2. Hill RM, Coates LC, Parmar PK, et al. 2002. Ann N Y Acad Sci, 971: 406-15. 3. Simonin Y, Charron Y, Sonderegger P, et al. 2006. J Neurosci, 26: 10614-9. 4. Ricagno S, Caccia S, Sorrentino G, et al. 2009. J Mol Biol, 388: 109-21. 5. Yepes MandLawrence DA. 2004. Thromb Haemost, 91: 457-64. |
| Background | Neuroserpin encoded by the SERPINI1 gene in humans is a member of the serine proteinase inhibitor (serpin) family. It reacts preferentially with tissue-type plasminogen activator (tPA). Neuroserpin is expressed mainly in the central nervous system (CNS) in response to neuronal depolarization and plays an important role in the development of synaptic plasticity. Mutations in human neuroserpin result in a form of autosomal dominant inherited dementia which is characterized by the presence of intraneuronal inclusion bodies and is known as Familial Encephalopathy with Neuroserpin Inclusion Bodies. |
