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  • 产品名称：重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白

  • 产品型号：rHusTNF RII/TNFRSF1B
  • 产品厂商：KALANG
  • 产品文档：
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简单介绍：
重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白与其它公司提供的重组蛋白不同，rHusTNF RII/TNFRSF1B蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：

重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白

Species Human
Accession P20333
GeneID 7133
Source Escherichia coli.
Molecular Weight 重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.
Quantity 5µg/20µg/1000µg
AA Sequence MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
Endotoxin 重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白Less than 0.1 EU/µg of rHusTNF RII/TNFRSF1B as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人可溶性肿瘤坏死因子受体II型/ TNFRSF1B蛋白
SDS-PAGE

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