产品详情
简单介绍：
重组人类4-1BB受体/ TNFRSF9蛋白与其它公司提供的重组蛋白不同，rHu4-1BB Receptor/TNFRSF9蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类4-1BB受体/ TNFRSF9蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类4-1BB受体/ TNFRSF9蛋白
| Synonyms | TNFRSF9, CD137 Antigen, T-cell Antigen ILA |
| Species | Human |
| Accession | Q07011 |
| GeneID | 3604 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类4-1BB受体/ TNFRSF9蛋白Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类4-1BB受体/ TNFRSF9蛋白Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 µg for both 4-1BB Ligand and 4-1BB Receptor. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl. |
| Endotoxin | 重组人类4-1BB受体/ TNFRSF9蛋白Less than 1 EU/μg of rHu4-1BB Receptor as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类4-1BB受体/ TNFRSF9蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65. 2. Barao I. 2012. Front Immunol, 3: 402. 3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27. 4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97. |
| Background | 4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. |
