产品详情
简单介绍：
重组人白细胞介素-36α，153a.a.蛋白与其它公司提供的重组蛋白不同，rHuIL-36α, 153a.a.蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人白细胞介素-36α，153a.a.蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人白细胞介素-36α，153a.a.蛋白
| Synonyms | FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1 |
| Species | Human |
| Accession | Q9UHA7 |
| GeneID | 27179 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人白细胞介素-36α，153a.a.蛋白Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人白细胞介素-36α，153a.a.蛋白Fully biologically active when compared to standard. The ED50as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1 % Tween 80. |
| Endotoxin | Less than 1 EU/μg of rHuIL-36α, 153a.a. as determined by LAL method. |
| Reconstitution | 重组人白细胞介素-36α，153a.a.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at - 20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人白细胞介素-36α，153a.a.蛋白 | |
| Reference | 1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25. 2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973. 3. Barksby HE, Lea SR, Preshaw PM, et al. 2007. Clin Exp Immunol. 149:217-25. 4. Smith DE, Renshaw BR, Ketchem RR, et al. 2000. J Biol Chem. 275:1169-75. 5. Dunn E, Sims JE, Nicklin MJ, et al. 2001. Trends Immunol. 22:533-6. |
| Background | Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha (153 a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57 – 68 % a.a. sequence identity with mouse, rabbit, equine and bovine IL-36α and 27 – 57 % a.a. sequence identity with other novel IL-1 family members. |
