产品详情
简单介绍：
重组人白细胞介素-19蛋白与其它公司提供的重组蛋白不同，rHuIL-19蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人白细胞介素-19蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人白细胞介素-19蛋白
| Synonyms | Melanoma Differentiation-associated Protein-like Protein, NG.1 |
| Species | Human |
| Accession | Q9UHD0 |
| GeneID | 29949 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人白细胞介素-19蛋白Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人白细胞介素-19蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重组人白细胞介素-19蛋白Less than 1 EU/µg of rHuIL-19 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人白细胞介素-19蛋白 | |
| Reference | 1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13. 2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50. 3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97. |
| Background | Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis. |
