产品详情
简单介绍：
重组人类单核细胞趋化蛋白-2 / CCL8蛋白与其它公司提供的重组蛋白不同，rHuMCP-2/CCL8蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类单核细胞趋化蛋白-2 / CCL8蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类单核细胞趋化蛋白-2 / CCL8蛋白
| Synonyms | HC14, Small-inducible cytokine A8 |
| Species | Human |
| Accession | P80075 |
| GeneID | 6355 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类单核细胞趋化蛋白-2 / CCL8蛋白Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | 重组人类单核细胞趋化蛋白-2 / CCL8蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重组人类单核细胞趋化蛋白-2 / CCL8蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类单核细胞趋化蛋白-2 / CCL8蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42. 3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74. 4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92. |
| Background | Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells. |
