产品详情
简单介绍：
重组人类BCA-1 / CXCL13蛋白与其它公司提供的重组蛋白不同，rHuBCA-1/CXCL13蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类BCA-1 / CXCL13蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类BCA-1 / CXCL13蛋白
| Synonyms | Angie, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13 |
| Species | Human |
| Accession | O43927 |
| GeneID | 10563 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类BCA-1 / CXCL13蛋白Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance | 重组人类BCA-1 / CXCL13蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuBCA-1/CXCL13 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重组人类BCA-1 / CXCL13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类BCA-1 / CXCL13蛋白 | |
| Reference | 1. Legler DF, Loetscher M, Roos RS, et al. 1998. J Exp Med. 187:655-60. 2. Gunn MD, Ngo VN, Ansel KM, et al. 1998. Nature. 391:799-803. 3. Ansel KM, Harris RB, Cyster JG. 2002. Immunity. 16:67-76. 4. Ansel KM, Ngo VN, Hyman PL, et al. 2000. Nature. 406:309-14. |
| Background | C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23–34 % amino acid sequence identity with other known CXC chemokines. |
