产品详情
简单介绍：
重组人类巨噬细胞炎性含有/ CCL18蛋白与其它公司提供的重组蛋白不同，rHuMIP-4/CCL18蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类巨噬细胞炎性含有/ CCL18蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类巨噬细胞炎性含有/ CCL18蛋白
| Synonyms | AMAC-1, PARC, DC-CK1 |
| Species | Human |
| Accession | P55774 |
| GeneID | 6362 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类巨噬细胞炎性含有/ CCL18蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类巨噬细胞炎性含有/ CCL18蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMIP-4/CCL18 as determined by LAL method. |
| Reconstitution | 重组人类巨噬细胞炎性含有/ CCL18蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类巨噬细胞炎性含有/ CCL18蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9. 2. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37. 3. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58. 4. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7. |
| Background | Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses. |
