产品详情
简单介绍：
重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白与其它公司提供的重组蛋白不同，rHuPTH1-84, 15N蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白
| Synonyms | Parathormone, Parathyrin |
| Species | Human |
| Accession | P01270 |
| GeneID | 5741 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白Approximately 9551 Da, a single non-glycosylated polypeptide chain containing 84 amino acids. 15N stable isotope labeled. |
| Quantity | 10µg/50µg/1000µg |
| AA Sequence | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuPTH1-84, 15N as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人甲状旁腺素1 - 84、15 n稳定同位素标记蛋白 | |
| Reference | 1. Potts, J.T. 2005. J Endocrinol, 187: 311-25. 2. Misiorowski, W. 2011. Endokrynol Pol, 62: 73-8. 3. Scillitani, A., V. Guarnieri, C. Battista, et al. 2011. J Endocrinol Invest, 34: 23-6. |
| Background | Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. PTH is a critical hormone in the regulation of Ca2+ homeostasis. PTH is secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. |
