产品详情
简单介绍：
重组人类乳腺丝氨酸蛋白酶抑制剂蛋白与其它公司提供的重组蛋白不同，rHuMaspin蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类乳腺丝氨酸蛋白酶抑制剂蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类乳腺丝氨酸蛋白酶抑制剂蛋白
| Synonyms | SerpinB5, Protease Inhibitor 5 |
| Species | Human |
| Accession | P36952 |
| GeneID | 5268 |
| Source | 重组人类乳腺丝氨酸蛋白酶抑制剂蛋白Escherichia coli. |
| Molecular Weight | Approximately 42.1 kDa, a single non-glycosylated polypeptide chain containing 375 amino acid residues. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | 重组人类乳腺丝氨酸蛋白酶抑制剂蛋白MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED重组人类乳腺丝氨酸蛋白酶抑制剂蛋白TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuMaspin as determined by LAL method. |
| Reconstitution | 重组人类乳腺丝氨酸蛋白酶抑制剂蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类乳腺丝氨酸蛋白酶抑制剂蛋白 | |
| Reference | 1. Goulet B, Kennette W, Ablack A, et al. 2011. Lab Invest, 91: 1181-7. 2. Schwarz S, Ettl T, Kleinsasser N, et al. 2008. Oral Oncol, 44: 563-70. 3. Abd El-Wahed MM. 2005. J Egypt Natl Canc Inst, 17: 173-83. 4. Katakura H, Takenaka K, Nakagawa M, et al. 2006. Lung Cancer, 51: 323-8. |
| Background | Mammary serine protease inhibitor (Maspin), also known as serpin B5 is a non-inhibitory serpin that is encoded by the SERPINB5 gene in humans. The protein is expressed predominantly in normal mammary epithelial cells but at significantly reduced levels or absent in most breast carcinomas. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The maspin functions as tumor suppressor, blocking the growth, invasion, and metastatic properties of mammary tumors. This anti-tumor activity is achieved, in part, by the ability of maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells. |
