产品详情
简单介绍:
重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白与其它公司提供的重组蛋白不同,rHuApo-SAA1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍:
重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白
Species | Human |
Accession | P0DJI8 |
GeneID | 6288 |
Source | 重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白Escherichia coli. |
Molecular Weight | Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids. |
Quantity | 10µg/50µg/1000µg |
AA Sequence | 重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | 重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
重组体人Apolipoprotein-Serum淀粉样蛋白A1蛋白 | |
SDS-PAGE |