小鼠原纤维蛋白1(FBN1)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗小鼠FBN1抗体包被于酶标板上，实验时标本或标准品中的FBN1会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗小鼠FBN1抗体和辣根过氧化物酶标记的亲和素。抗小鼠FBN1抗体与结合在包被抗体上的小鼠FBN1结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），FBN1浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中FBN1的浓度。
小鼠原纤维蛋白1(FBN1)化学发光**分析试剂盒描述：
| 英文名称 | Mouse FBN1 (Fibrillin 1) CLIA Kit | ||
| 中文名称 | 小鼠原纤维蛋白1(FBN1)化学发光**分析试剂盒 | ||
| 货号 | KL-M0297c | 种属 | Mouse/小鼠 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 3.125~200pg/mL | 灵敏度 | 1.875pg/mL |
小鼠原纤维蛋白1(FBN1)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Manual | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| 特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
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1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-TNFb-Si 猴肿瘤坏死因子β(TNFβ) 检测试剂盒 Monkey Tumor Necrosis Factor Beta (TNFb) ELISA Kit Rhesus monkey Simian 15.625-1000 ng/mL 3.8 ng/mL 96T 12 months
KL-IGFBP3-b 牛胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 Bovine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Bos taurus; Bovine Cattle 62.5-4000pg/mL 23.5pg/mL 96T 12 months
KL-IGFBP3-g 山羊胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 Goat Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Capra hircus; Caprine Goat 3.125-200 ng/mL 0.214 ng/mL 96T 12 months
KL-IGFBP3-Eq 马胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 Equine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Equus caballus; Equine Horse 3.125-200 ng/mL 0.178 ng/mL 96T 12 months
KL-IGFBP3-Fi 鱼胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 fish Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Danio rerio Zebrafish 0.313-20 ng/mL 0.124 ng/mL 96T 12 months
KL-IGFBP3-Ch 鸡胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 Chicken Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Chicken Gallus 6.25-400 ng/mL 2.7 ng/mL 96T 12 months
KL-IGFBP4-b 牛胰岛素样生长因子结合蛋白4(IGFBP4) 检测试剂盒 Bovine Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit Bos taurus; Bovine Cattle 74.07-6000pg/mL 29.62pg/mL 96T 12 months
KL-IGFBP4-g 山羊胰岛素样生长因子结合蛋白4(IGFBP4) 检测试剂盒 Goat Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit Capra hircus; Caprine Goat 0.313-20 ng/mL 0.121 ng/mL 96T 12 months
KL-IGFBP4-Ch 鸡胰岛素样生长因子结合蛋白4(IGFBP4) 检测试剂盒 Chicken Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit Chicken Gallus 0.313-20 ng/mL 0.085 ng/mL 96T 12 months
KL-IGFBP4-p 猪胰岛素样生长因子结合蛋白4(IGFBP4) 检测试剂盒 porcine Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit Sus scrofa; Porcine Pig 0.313-20 ng/mL 0.098 ng/mL 96T 12 months
KL-COL1a2-b 牛Ⅰ型胶原α2(COL1α2) 检测试剂盒 Bovine Collagen Type I Alpha 2 (COL1a2) ELISA Kit Bos taurus; Bovine Cattle 0.313-20ng/mL 0.105ng/mL 96T 12 months
KL-PROC-Mu 小鼠蛋白C(PROC) 检测试剂盒 mouse Protein C (PROC) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 14.1pg/mL 96T 12 months
KL-PROC-Ra 大鼠蛋白C(PROC) 检测试剂盒 Rat Protein C (PROC) ELISA Kit Rattus norvegicus Rat 1.563-100 ng/mL 0.42 ng/mL 96T 12 months
KL-COL4a1-Hu 人Ⅳ型胶原α1(COL4α1) 检测试剂盒 human Collagen Type IV Alpha 1 (COL4a1) ELISA Kit Homo sapiens human 0.938-60ng/mL 0.45ng/mL 96T 12 months
KL-EVPL-Hu 人外皮蛋白(EVPL) 检测试剂盒 human Envoplakin (EVPL) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.064ng/mL 96T 12 months
KL-GTF2H1-Mu 小鼠通用转录因子ⅡH肽1(GTF2H1) 检测试剂盒 mouse General Transcription Factor IIH, Polypeptide 1 (GTF2H1) ELISA Kit Mus musculus mouse 0.313-20 ng/mL 0.16 ng/mL 96T 12 months
