小鼠细胞色素P450 7A1(CYP7A1)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗小鼠CYP7A1抗体包被于酶标板上，实验时标本或标准品中的CYP7A1会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗小鼠CYP7A1抗体和辣根过氧化物酶标记的亲和素。抗小鼠CYP7A1抗体与结合在包被抗体上的小鼠CYP7A1结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），CYP7A1浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中CYP7A1的浓度。
小鼠细胞色素P450 7A1(CYP7A1)化学发光**分析试剂盒描述：
| 英文名称 | Mouse CYP7A1 (Cytochrome P450 7A1) CLIA Kit | ||
| 中文名称 | 小鼠细胞色素P450 7A1(CYP7A1)化学发光**分析试剂盒 | ||
| 货号 | KL-M0251c | 种属 | Mouse/小鼠 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 62.5~4000pg/mL | 灵敏度 | 37.5pg/mL |
小鼠细胞色素P450 7A1(CYP7A1)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Manual | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| 特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
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1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
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KL-MUC5B-Mu 小鼠粘蛋白5B(MUC5B) 检测试剂盒 mouse Mucin 5 Subtype B (MUC5B) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 6.7pg/mL 96T 12 months
KL-GCG-Ch 鸡胰高血糖素(GC) 检测试剂盒 Chicken Glucagon (GC) ELISA Kit Chicken Gallus 18.52-1500pg/mL 7.25pg/mL 96T 12 months
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KL-IL8-Gu 豚鼠白介素8(IL8) 检测试剂盒 Guinea pig Interleukin 8 (IL8) ELISA Kit Cavia Guinea pig 15.625-1000pg/mL 6.8pg/mL 96T 12 months
KL-IL10-Gu 豚鼠白介素10(IL10) 检测试剂盒 Guinea pig Interleukin 10 (IL10) ELISA Kit Cavia Guinea pig 15.625-1000pg/mL 5.2pg/mL 96T 12 months
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KL-IDE-Mu 小鼠胰岛素降解酶(IDE) 检测试剂盒 mouse Insulin Degrading Enzyme (IDE) ELISA Kit Mus musculus mouse 3.125-200pg/mL 1.33pg/mL 96T 12 months
KL-aLA-b 牛α-乳清蛋白(αLA) 检测试剂盒 Bovine Alpha-Lactalbumin (aLA) ELISA Kit Bos taurus; Bovine Cattle 0.781-50ng/mL 0.32ng/mL 96T 12 months
KL-Ub-Ra 大鼠泛素(Ub) 检测试剂盒 Rat Ubiquitin (Ub) ELISA Kit Rattus norvegicus Rat 62.5-4000 pg/mL 15.6 pg/mL 96T 12 months
KL-IL17-c 犬白介素17(IL17) 检测试剂盒 Canine Interleukin 17 (IL17) ELISA Kit Canis familiaris; Canine Dog 7.813-500pg/mL 3.1pg/mL 96T 12 months
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KL-ProIAPP-Hu 人前胰淀粉样蛋白(ProIAPP) 检测试剂盒 human Proislet Amyloid Polypeptide (ProIAPP) ELISA Kit Homo sapiens human 24.69-2000pg/mL 10.15pg/mL 96T 12 months
KL-TLR4-Mu 小鼠Toll样受体4(TLR4) 检测试剂盒 mouse Toll Like Receptor 4 (TLR4) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.065ng/mL 96T 12 months
