大鼠超敏C反应蛋白(hs-CRP)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗大鼠hs-CRP抗体包被于酶标板上，实验时标本或标准品中的hs-CRP会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠hs-CRP抗体和辣根过氧化物酶标记的亲和素。抗大鼠hs-CRP抗体与结合在包被抗体上的大鼠hs-CRP结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），hs-CRP浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中hs-CRP的浓度。
大鼠超敏C反应蛋白(hs-CRP)化学发光**分析试剂盒描述：
| 英文名称 | Rat hs-CRP (High Sensitivity C-Reactive Protein) CLIA Kit | ||
| 中文名称 | 大鼠超敏C反应蛋白(hs-CRP)化学发光**分析试剂盒 | ||
| 货号 | KL-R0349c | 种属 | Rat/大鼠 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 31.25~2000pg/mL | 灵敏度 | 18.75pg/mL |
大鼠超敏C反应蛋白(hs-CRP)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Manual | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| 特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
|||
1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-TIMP2-Fi 鱼组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 fish Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Danio rerio Zebrafish 1.563-100ng/mL 0.39ng/mL 96T 12 months
KL-TIMP2-Ch 鸡组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 Chicken Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Chicken Gallus 7.813-500ng/mL 1.78ng/mL 96T 12 months
KL-TIMP2-Gu 豚鼠组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 Guinea pig Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Cavia Guinea pig 1.563-100ng/mL 0.38ng/mL 96T 12 months
KL-TIMP2-p 猪组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 porcine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Sus scrofa; Porcine Pig 0.313-20ng/mL 0.103ng/mL 96T 12 months
KL-TIMP2-Rb 兔组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 Rabbit Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Oryctolagus cuniculus Rabbit 7.813-500ng/mL 1.92ng/mL 96T 12 months
KL-TIMP3-b 牛组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Bovine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Bos taurus; Bovine Cattle 0.313-20ng/mL 0.118ng/mL 96T 12 months
KL-TIMP3-g 山羊组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Goat Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Capra hircus; Caprine Goat 0.313-20ng/mL 0.128ng/mL 96T 12 months
KL-TIMP3-Eq 马组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Equus caballus; Equine Horse 0.313-20ng/mL 0.147ng/mL 96T 12 months
KL-TIMP3-Fi 鱼组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 fish Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Danio rerio Zebrafish 0.781-50ng/mL 0.194ng/mL 96T 12 months
KL-TIMP3-Ch 鸡组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Chicken Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Chicken Gallus 0.156-10ng/mL 0.076ng/mL 96T 12 months
KL-TIMP3-p 猪组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 porcine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Sus scrofa; Porcine Pig 0.156-10ng/mL 0.052ng/mL 96T 12 months
KL-TIMP3-Rb 兔组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Rabbit Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Oryctolagus cuniculus Rabbit 0.313-20ng/mL 0.087ng/mL 96T 12 months
KL-TIMP3-Si 猴组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Monkey Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Rhesus monkey Simian 0.313-20ng/mL 0.108ng/mL 96T 12 months
KL-MUC6-Hu 人粘蛋白6(MUC6) 检测试剂盒 human Mucin 6 (MUC6) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.115ng/mL 96T 12 months
KL-SYNPO-Hu 人突触足蛋白(SYNPO) 检测试剂盒 human Synaptopodin (SYNPO) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.4pg/mL 96T 12 months
