大鼠血栓调节蛋白(TM)化学发光**分析试剂盒
基本原理:
本试剂盒采用双抗体夹心法。用抗大鼠TM抗体包被于酶标板上,实验时标本或标准品中的TM会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗大鼠TM抗体和辣根过氧化物酶标记的亲和素。抗大鼠TM抗体与结合在包被抗体上的大鼠TM结合、生物素与亲和素特异性结合而形成**复合物,游离的成分被洗去。加入发光底物混合液,发光底物在辣根过氧化物酶的催化下发出荧光,用化学发光**分析仪测定化学发光值(RLU),TM浓度与化学发光值之间呈正相关,通过绘制标准曲线求出标本中TM的浓度。
大鼠血栓调节蛋白(TM)化学发光**分析试剂盒描述:
英文名称 | Rat TM (Thrombomodulin) CLIA Kit | ||
中文名称 | 大鼠血栓调节蛋白(TM)化学发光**分析试剂盒 | ||
货号 | KL-R0638c | 种属 | Rat/大鼠 |
规格 | 96T/Kit (8*12 wells) | ||
检测方法 | 双抗体夹心法 | ||
检测范围 | 15.625~1000pg/mL | 灵敏度 | 9.375pg/mL |
大鼠血栓调节蛋白(TM)化学发光**分析试剂盒试剂盒组成:
中文名称 | 英文名称 | 规格 | 保存 |
CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Manual | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 | |
特别说明: #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
1.在各孔中加入标准品或样品各 100μL,37℃孵育90分钟
2. 倒去孔内液体,拍干,加入 100μL 生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液, 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-TPO-Mu 小鼠血小板生成素(TPO) 检测试剂盒 mouse Thrombopoietin (TPO) ELISA Kit Mus musculus mouse 7.813-500pg/mL 3.2pg/mL 96T 12 months
KL-TNFb-Ra 大鼠肿瘤坏死因子β(TNFβ) 检测试剂盒 Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit Rattus norvegicus Rat 15.625-1000pg/mL 5.7pg/mL 96T 12 months
KL-TIMP4-Ra 大鼠组织金属蛋白酶抑制因子4(TIMP4) 检测试剂盒 Rat Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit Rattus norvegicus Rat 78.125-5000pg/mL 24pg/mL 96T 12 months
KL-TIMP4-Mu 小鼠组织金属蛋白酶抑制因子4(TIMP4) 检测试剂盒 mouse Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 32pg/mL 96T 12 months
KL-TIMP3-Ra 大鼠组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 Rat Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.054ng/mL 96T 12 months
KL-TIMP3-Mu 小鼠组织金属蛋白酶抑制因子3(TIMP3) 检测试剂盒 mouse Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.051ng/mL 96T 12 months
KL-TIMP2-Ra 大鼠组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Rattus norvegicus Rat 1.563-100ng/mL 0.60ng/mL 96T 12 months
KL-TIMP2-Mu 小鼠组织金属蛋白酶抑制因子2(TIMP2) 检测试剂盒 mouse Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit Mus musculus mouse 0.313-20ng/mL 0.131ng/mL 96T 12 months
KL-Tie2-Ra 大鼠内皮细胞TEK酪氨酸激酶(Tie2) 检测试剂盒 Rat TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.133ng/mL 96T 12 months
KL-Tie1-Ra 大鼠**球蛋白样EGF样域酪氨酸激酶1(Tie1) 检测试剂盒 Rat Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit Rattus norvegicus Rat 7.813-500 ng/mL 1.98 ng/mL 96T 12 months
KL-Tie1-Mu 小鼠**球蛋白样EGF样域酪氨酸激酶1(Tie1) 检测试剂盒 mouse Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit Mus musculus mouse 0.625-40ng/mL 0.247ng/mL 96T 12 months
KL-SDF1-Ra 大鼠基质细胞衍生因子1(SDF1) 检测试剂盒 Rat Stromal Cell Derived Factor 1 (SDF1) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.125ng/mL 96T 12 months