大鼠尿激酶型纤溶酶原激活因子(PLAU/uPA)化学发光**分析试剂盒
基本原理:
本试剂盒采用双抗体夹心法。用抗大鼠PLAU/uPA抗体包被于酶标板上,实验时标本或标准品中的PLAU/uPA会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗大鼠PLAU/uPA抗体和辣根过氧化物酶标记的亲和素。抗大鼠PLAU/uPA抗体与结合在包被抗体上的大鼠PLAU/uPA结合、生物素与亲和素特异性结合而形成**复合物,游离的成分被洗去。加入发光底物混合液,发光底物在辣根过氧化物酶的催化下发出荧光,用化学发光**分析仪测定化学发光值(RLU),PLAU/uPA浓度与化学发光值之间呈正相关,通过绘制标准曲线求出标本中PLAU/uPA的浓度。
大鼠尿激酶型纤溶酶原激活因子(PLAU/uPA)化学发光**分析试剂盒描述:
英文名称 | Rat PLAU/uPA(Urokinase-Type Plasminogen Activator) CLIA Kit | ||
中文名称 | 大鼠尿激酶型纤溶酶原激活因子(PLAU/uPA)化学发光**分析试剂盒 | ||
货号 | KL-R0682c | 种属 | Rat/大鼠 |
规格 | 96T/Kit (8*12 wells) | ||
检测方法 | 双抗体夹心法 | ||
检测范围 | 7.813~500pg/mL | 灵敏度 | 4.688pg/mL |
大鼠尿激酶型纤溶酶原激活因子(PLAU/uPA)化学发光**分析试剂盒试剂盒组成:
中文名称 | 英文名称 | 规格 | 保存 |
CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Manual | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 | |
特别说明: #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
1.在各孔中加入标准品或样品各 100μL,37℃孵育90分钟
2. 倒去孔内液体,拍干,加入 100μL 生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液, 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-NPY-b 牛神经肽Y(NPY) 检测试剂盒 Bovine Neuropeptide Y (NPY) ELISA Kit Bos taurus; Bovine Cattle 24.69-2000pg/mL 8.52pg/mL 96T 12 months
KL-IL12A-Ch 鸡白介素12A(IL12A) 检测试剂盒 Chicken Interleukin 12A (IL12A) ELISA Kit Chicken Gallus 15.625-1000pg/mL 7.2pg/mL 96T 12 months
KL-APOB-p 猪载脂蛋白B(APOB) 检测试剂盒 porcine Apolipoprotein B (APOB) ELISA Kit Sus scrofa; Porcine Pig 62.5-4000ng/mL 23.5ng/mL 96T 12 months
KL-LAT1-Hu 人大型中性氨基酸转运蛋白1(LAT1) 检测试剂盒 human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.056ng/mL 96T 12 months
KL-IL8Rb-Ra 大鼠白介素8受体β(IL8Rβ) 检测试剂盒 Rat Interleukin 8 Receptor Beta (IL8Rb) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.109ng/mL 96T 12 months
KL-IL34-Hu 人白介素34(IL34) 检测试剂盒 human Interleukin 34 (IL34) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.9pg/mL 96T 12 months
KL-IL34-Mu 小鼠白介素34(IL34) 检测试剂盒 mouse Interleukin 34 (IL34) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.3pg/mL 96T 12 months
KL-Dyn-Mu 小鼠强啡肽(Dyn) 检测试剂盒 mouse Big Dynorphin (Dyn) ELISA Kit Mus musculus mouse 3.7-300pg/mL 1.35pg/mL 96T 12 months
KL-ENK-Mu 小鼠脑啡肽(ENK) 检测试剂盒 mouse Enkephalin (ENK) ELISA Kit Mus musculus mouse 74.07-6000pg/mL 25.35pg/mL 96T 12 months
KL-IL24-Hu 人白介素24(IL24) 检测试剂盒 human Interleukin 24 (IL24) ELISA Kit Homo sapiens human 7.813-500pg/mL 2.7pg/mL 96T 12 months
KL-MT1-Mu 小鼠金属硫蛋白1(MT1) 检测试剂盒 mouse Metallothionein 1 (MT1) ELISA Kit Mus musculus mouse 62.5-4000pg/mL 35.8pg/mL 96T 12 months
KL-MSTN-Mu 小鼠肌肉生长抑制素(MSTN) 检测试剂盒 mouse Myostatin (MSTN) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 35pg/mL 96T 12 months
KL-CA2-Ch 鸡碳酸酐酶Ⅱ(CA2) 检测试剂盒 Chicken Carbonic Anhydrase II (CA2) ELISA Kit Chicken Gallus 1.563-100ng/mL 0.059ng/mL 96T 12 months
KL-aHSG-Mu 小鼠α2-HS糖蛋白(αHSG) 检测试剂盒 mouse Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit Mus musculus mouse 9.375-600ng/mL 3.64ng/mL 96T 12 months
KL-RANkL-Ch 鸡核因子κB受体激活因子配基(RANκL) 检测试剂盒 Chicken Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit Chicken Gallus 7.813-500 pg/mL 2.5 pg/mL 96T 12 months