人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)化学发光**分析试剂盒
基本原理:
本试剂盒采用双抗体夹心法。用抗人IGF2BP3抗体包被于酶标板上,实验时标本或标准品中的IGF2BP3会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IGF2BP3抗体和辣根过氧化物酶标记的亲和素。抗人IGF2BP3抗体与结合在包被抗体上的人IGF2BP3结合、生物素与亲和素特异性结合而形成**复合物,游离的成分被洗去。加入发光底物混合液,发光底物在辣根过氧化物酶的催化下发出荧光,用化学发光**分析仪测定化学发光值(RLU),IGF2BP3浓度与化学发光值之间呈正相关,通过绘制标准曲线求出标本中IGF2BP3的浓度。
人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)化学发光**分析试剂盒描述:
英文名称 | Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) CLIA Kit | ||
中文名称 | 人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)化学发光**分析试剂盒 | ||
货号 | KL-H0357c | 种属 | Human/人 |
规格 | 96T/Kit (8*12 wells) | ||
检测方法 | 双抗体夹心法 | ||
检测范围 | 62.5~4000pg/mL | 灵敏度 | 37.5pg/mL |
人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)化学发光**分析试剂盒试剂盒组成:
中文名称 | 英文名称 | 规格 | 保存 |
CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Manual | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 | |
特别说明: #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
1.在各孔中加入标准品或样品各 100μL,37℃孵育90分钟
2. 倒去孔内液体,拍干,加入 100μL 生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液, 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-IGFBP3-Mu 小鼠胰岛素样生长因子结合蛋白3(IGFBP3) 检测试剂盒 mouse Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit Mus musculus mouse 4.688-300ng/mL 2.0ng/mL 96T 12 months
KL-IGFBP4-Hu 人胰岛素样生长因子结合蛋白4(IGFBP4) 检测试剂盒 human Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit Homo sapiens human 3.125-200ng/mL 1.13ng/mL 96T 12 months
KL-IL10-Hu 人白介素10(IL10) 检测试剂盒 human Interleukin 10 (IL10) ELISA Kit Homo sapiens human 31.25-2000pg/mL 12.3pg/mL 96T 12 months
KL-IL10-Mu 小鼠白介素10(IL10) 检测试剂盒 mouse Interleukin 10 (IL10) ELISA Kit Mus musculus mouse 7.813-500pg/mL 2.5pg/mL 96T 12 months
KL-IL10-Ra 大鼠白介素10(IL10) 检测试剂盒 Rat Interleukin 10 (IL10) ELISA Kit Rattus norvegicus Rat 7.813-500pg/mL 2.7pg/mL 96T 12 months
KL-IL11-Hu 人白介素11(IL11) 检测试剂盒 human Interleukin 11 (IL11) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.8pg/mL 96T 12 months
KL-IL11-Mu 小鼠白介素11(IL11) 检测试剂盒 mouse Interleukin 11 (IL11) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 7.1pg/mL 96T 12 months
KL-IL12B-Hu 人白介素12B(IL12B) 检测试剂盒 human Interleukin 12B (IL12B) ELISA Kit Homo sapiens human 7.813-500pg/mL 3.2pg/mL 96T 12 months
KL-IL12A-Hu 人白介素12A(IL12A) 检测试剂盒 human Interleukin 12A (IL12A) ELISA Kit Homo sapiens human 7.813-500pg/mL 2.7pg/mL 96T 12 months
KL-IL12A-Mu 小鼠白介素12A(IL12A) 检测试剂盒 mouse Interleukin 12A (IL12A) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.7pg/mL 96T 12 months
KL-IL13-Hu 人白介素13(IL13) 检测试剂盒 human Interleukin 13 (IL13) ELISA Kit Homo sapiens human 15.625-1000pg/mL 7.1pg/mL 96T 12 months
KL-IL13-Mu 小鼠白介素13(IL13) 检测试剂盒 mouse Interleukin 13 (IL13) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 5.8pg/mL 96T 12 months
KL-IL15-Hu 人白介素15(IL15) 检测试剂盒 human Interleukin 15 (IL15) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.8pg/mL 96T 12 months
KL-IL15-Mu 小鼠白介素15(IL15) 检测试剂盒 mouse Interleukin 15 (IL15) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 6.1pg/mL 96T 12 months