产品详情
简单介绍：
重组小鼠Lungkine / CXCL15蛋白与其它公司提供的重组蛋白不同，rMuLungkine/CXCL15蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠Lungkine / CXCL15蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组小鼠Lungkine / CXCL15蛋白
| Species | Murine |
| Accession | Q9WVL7 |
| GeneID | 20309 |
| Unigene | Mm.64326. |
| Source | Escherichia coli. |
| Molecular Weight | 重组小鼠Lungkine / CXCL15蛋白Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 142 amino acids. |
| AA Sequence | QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组小鼠Lungkine / CXCL15蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20. |
| Endotoxin | Less than 1 EU/µg of rMuLungkine/CXCL15 as determined by LAL method. |
| Reconstitution | 重组小鼠Lungkine / CXCL15蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组小鼠Lungkine / CXCL15蛋白 | |
| Background | Mouse Lungkine/CXCL15, also named WECHE, is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids (aa) with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the ***** and fetal lung, and its expression is up-regulated under inflammatory conditions. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace. Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation. |
