产品详情
简单介绍：
重组人类Cu / Zn超氧化物歧化酶蛋白与其它公司提供的重组蛋白不同，rHuCu/Zn SOD, His蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类Cu / Zn超氧化物歧化酶蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类Cu / Zn超氧化物歧化酶蛋白
| Synonyms | SOD1 |
| Species | Human |
| Accession | P00441 |
| GeneID | 6647 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类Cu / Zn超氧化物歧化酶蛋白Approximately 39.9 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 × 189 amino acids with Met, Gly and 10 × His at N-terminus. |
| Quantity | 20µg/100µg/1000µg |
| AA Sequence | MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQ |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类Cu / Zn超氧化物歧化酶蛋白Fully biologically active when compared to standard. The potency per mg was determined by Pyrogallic Acid method and was found to be more than 1.0 × 104IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuCu/Zn SOD, His as determined by LAL method. |
| Reconstitution | 重组人类Cu / Zn超氧化物歧化酶蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类Cu / Zn超氧化物歧化酶蛋白 | |
| Reference | 1. Kwiatowski J, Skarecky D, Ayala FJ. 1992. Mol Phylogenet Evol, 1: 72-82. 2. Bachus R, Claus A, Megow D, et al. 1995. J Neurol Sci, 129 Suppl: 93-5. 3. Qi X, Guy J, Nick H, et al. 1997. Invest Ophthalmol Vis Sci, 38: 1203-12. 4. Chou CM, Huang CJ, Shih CM, et al. 2005. Ann N Y Acad Sci, 1042: 303-13. 5. Raja SB, Murali MR, Roopa K, et al. 2011. Biomed Pharmacother, 65: 560-8. |
| Background | Superoxide dismutase catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. Cu/Zn superoxide dismutase also named as SOD1, is an enzyme encoded by the SOD1 gene in humans, located on chromosome 21. The SOD1 binds Cu and Zn ions and is one of three SODs responsible for destroying free superoxide radicals in the body. It has been shown to interact with CCS and Bcl-2. The malfunction of SOD1 may increase the risk of illnesses like age-related muscle mass loss (sarcopenia), early development of cataracts, macular degeneration, thymic involution, hepatocellular carcinoma, shortened lifespan, keratoconus and amyotrophic lateral sclerosis. |
