产品详情
简单介绍：
重组人类B细胞成熟抗原/ TNFRSF17蛋白与其它公司提供的重组蛋白不同，rHuBCMA/TNFRSF17蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类B细胞成熟抗原/ TNFRSF17蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类B细胞成熟抗原/ TNFRSF17蛋白
| Synonyms | TNFRSF17, BCM, CD269 |
| Species | Human |
| Accession | Q02223 |
| GeneID | 608 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类B细胞成熟抗原/ TNFRSF17蛋白Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类B细胞成熟抗原/ TNFRSF17蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104IU/mg in the presence of 100 ng/ml human APRIL. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
| Endotoxin | 重组人类B细胞成熟抗原/ TNFRSF17蛋白Less than 1 EU/μg of rHuBCMA as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类B细胞成熟抗原/ TNFRSF17蛋白 | |
| Reference | 1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81. 2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62. 3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33. 4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54. |
| Background | B-Cell Maturation Antigen (BCMA), encoded by the TNFRSF17 gene in humans. It is a member of the TNF receptor superfamily and a type III membrane protein containing one extracellular cysteine rich domain. It is expressed on mature B-cells and other B-cell lines. BCMA can binds to TNFSF13B/BLyS/BAFF and TNFSF13/APRIL, and has functions that promotes B-cell survival, plays a role in the regulation of humoral immunity, and activates NF-kappa-B and JNK BCMA. |
