产品详情
简单介绍：
核因子κ-B的重组人可溶性受体激活剂蛋白与其它公司提供的重组蛋白不同，rHusRANK Receptor/TNFRSF11A蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.核因子κ-B的重组人可溶性受体激活剂蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
核因子κ-B的重组人可溶性受体激活剂蛋白
| Species | Human |
| Accession | Q9Y6Q6 |
| GeneID | 8792 |
| Source | Escherichia coli. |
| Molecular Weight | 核因子κ-B的重组人可溶性受体激活剂蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Quantity | 20µg/100µg/1000µg |
| AA Sequence | QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 核因子κ-B的重组人可溶性受体激活剂蛋白Fully biologically active when compared to standard. The ED50as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 核因子κ-B的重组人可溶性受体激活剂蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl. |
| Endotoxin | Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 核因子κ-B的重组人可溶性受体激活剂蛋白 | |
| SDS-PAGE |  |
