产品详情
简单介绍：
干扰素-γ/ CXCL9诱导的重组人单核因子蛋白与其它公司提供的重组蛋白不同，rHuIP-10/CXCL10蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.干扰素-γ/ CXCL9诱导的重组人单核因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
干扰素-γ/ CXCL9诱导的重组人单核因子蛋白
| Synonyms | Gamma-IP10, Small-inducible Cytokine B10 |
| Species | Human |
| Accession | P02778 |
| GeneID | 3627 |
| Source | Escherichia coli. |
| Molecular Weight | 干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 干扰素-γ/ CXCL9诱导的重组人单核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Endotoxin | Less than 1 EU/µg of rHuIP-10/CXCL10 as determined by LAL method. |
| Reconstitution | 干扰素-γ/ CXCL9诱导的重组人单核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 干扰素-γ/ CXCL9诱导的重组人单核因子蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6. 2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71. 3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25. 4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204. 5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62. |
| Background | CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10. |
