产品详情
简单介绍：
重组人类血液滤液CC趋化因子-1 / CCL14蛋白与其它公司提供的重组蛋白不同，rHuHCC-1/CCL14蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类血液滤液CC趋化因子-1 / CCL14蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人类血液滤液CC趋化因子-1 / CCL14蛋白
| Synonyms | HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14 |
| Species | Human |
| Accession | Q16627 |
| GeneID | 6358 |
| Source | Escherichia coli. |
| Molecular Weight | 重组人类血液滤液CC趋化因子-1 / CCL14蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人类血液滤液CC趋化因子-1 / CCL14蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuHCC-1/CCL14 as determined by LAL method. |
| Reconstitution | 重组人类血液滤液CC趋化因子-1 / CCL14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人类血液滤液CC趋化因子-1 / CCL14蛋白 | |
| Reference | 1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9. 2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8. 3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13. |
| Background | Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma. |
