产品详情
简单介绍：
重组人中脑的Astrocyte-Derived神经营养因子蛋白与其它公司提供的重组蛋白不同，rHuMANF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人中脑的Astrocyte-Derived神经营养因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人中脑的Astrocyte-Derived神经营养因子蛋白
| Synonyms | ARMET |
| Species | Human |
| Accession | P55145 |
| GeneID | 7873 |
| Source | 重组人中脑的Astrocyte-Derived神经营养因子蛋白Escherichia coli. |
| Molecular Weight | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组人中脑的Astrocyte-Derived神经营养因子蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rHuMANF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重组人中脑的Astrocyte-Derived神经营养因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人中脑的Astrocyte-Derived神经营养因子蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88. 2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13. 3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71. |
| Background | MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively. |
