产品详情
简单介绍：
重组人胰岛素样生长因子- 1蛋白与其它公司提供的重组蛋白不同，rHuIGF-1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人胰岛素样生长因子- 1蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组人胰岛素样生长因子- 1蛋白
| Synonyms | Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF |
| Species | Human |
| Accession | 重组人胰岛素样生长因子- 1蛋白P05019 |
| GeneID | 3479 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 100µg/500µg/1000µg |
| AA Sequence | 重组人胰岛素样生长因子- 1蛋白GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Assay #1: Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106IU/mg. Assay #2: Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105IU/mg. |
| Physical Appearance | 重组人胰岛素样生长因子- 1蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Endotoxin | Less than 0.01 EU/μg of rHuIGF-1 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable for 3 years at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to two weeks at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组人胰岛素样生长因子- 1蛋白 | |
| SDS-PAGE |  |
