大鼠线粒体肌酸激酶(CKMT1B)化学发光**分析试剂盒
基本原理：
本试剂盒采用双抗体夹心法。用抗大鼠CKMT1B抗体包被于酶标板上，实验时标本或标准品中的CKMT1B会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠CKMT1B抗体和辣根过氧化物酶标记的亲和素。抗大鼠CKMT1B抗体与结合在包被抗体上的大鼠CKMT1B结合、生物素与亲和素特异性结合而形成**复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光**分析仪测定化学发光值（RLU），CKMT1B浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中CKMT1B的浓度。
大鼠线粒体肌酸激酶(CKMT1B)化学发光**分析试剂盒描述：
| 英文名称 | Rat CKMT1B (Creatine Kinase 1B, Mitochondrial) CLIA Kit | ||
| 中文名称 | 大鼠线粒体肌酸激酶(CKMT1B)化学发光**分析试剂盒 | ||
| 货号 | KL-R0180c | 种属 | Rat/大鼠 |
| 规格 | 96T/Kit (8*12 wells) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 0.625~40ng/mL | 灵敏度 | 0.375ng/mL |
大鼠线粒体肌酸激酶(CKMT1B)化学发光**分析试剂盒试剂盒组成：
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
| 浓缩洗涤液（25×） | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Manual | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| 特别说明： #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃. |
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1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液， 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
KL-CA9-Mu 小鼠碳酸酐酶Ⅸ(CA9) 检测试剂盒 mouse Carbonic Anhydrase IX (CA9) ELISA Kit Mus musculus mouse 1.563-100pg/mL 0.61pg/mL 96T 12 months
KL-TAS1R2-Mu 小鼠味觉受体1型成员2(TAS1R2) 检测试剂盒 mouse Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit Mus musculus mouse 0.156-10 ng/mL 0.043 ng/mL 96T 12 months
KL-NEFL-Hu 人轻肽神经丝蛋白(NEFL) 检测试剂盒 human Neurofilament, Light Polypeptide (NEFL) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.9pg/mL 96T 12 months
KL-F12-Mu 小鼠凝血因子Ⅻ(F12) 检测试剂盒 mouse Coagulation Factor XII (F12) ELISA Kit Mus musculus mouse 0.625-40ng/mL 0.251ng/mL 96T 12 months
KL-GSTa3-Ra 大鼠谷胱甘肽S转移酶α3(GSTα3) 检测试剂盒 Rat Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.079ng/mL 96T 12 months
KL-FceRII-Mu 小鼠IgE-Fc片段受体Ⅱ(FcεRⅡ) 检测试剂盒 mouse Receptor II For The Fc Region Of Immunoglobulin E (FceRII) ELISA Kit Mus musculus mouse 1.563-100pg/mL 0.66pg/mL 96T 12 months
KL-TPH1-Ra 大鼠色胺酸羟化酶1(TPH1) 检测试剂盒 Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.116ng/mL 96T 12 months
KL-RBP1-Hu 人视黄醇结合蛋白1(RBP1) 检测试剂盒 human Retinol Binding Protein 1, Cellular (RBP1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.061ng/mL 96T 12 months
KL-GNb1-Mu 小鼠G蛋白β1(GNβ1) 检测试剂盒 mouse G Protein Beta 1 (GNb1) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.041ng/mL 96T 12 months
KL-TGFbR2-Hu 人转化生长因子β受体Ⅱ(TGFβR2) 检测试剂盒 human Transforming Growth Factor Beta Receptor II (TGFbR2) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.126ng/mL. 96T 12 months
KL-SQSTM1-Hu 人死骨片1(SQSTM1) 检测试剂盒 human Sequestosome 1 (SQSTM1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.125ng/mL 96T 12 months
KL-GIF-Hu 人胃内因子(GIF) 检测试剂盒 human Gastric Intrinsic Factor (GIF) ELISA Kit Homo sapiens human 2.47-200ng/mL 0.95ng/mL 96T 12 months
KL-PCSK1-Hu 人前蛋白转化酶枯草溶菌素1(PCSK1) 检测试剂盒 human Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.101ng/mL 96T 12 months
KL-CRABP2-Hu 人细胞维甲酸结合蛋白2(CRABP2) 检测试剂盒 human Cellular Retinoic Acid Binding Protein 2 (CRABP2) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.114ng/mL 96T 12 months
KL-KIF1A-Hu 人驱动蛋白家族成员1A(KIF1A) 检测试剂盒 human Kinesin Family, Member 1A (KIF1A) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.051ng/mL 96T 12 months
