人I类主要组织相容性复合体E(MHCE/HLA-E)化学发光**分析试剂盒
基本原理:
本试剂盒人I类主要组织相容性复合体E(MHCE/HLA-E)化学发光**分析试剂盒
人I类主要组织相容性复合体E(MHCE/HLA-E)化学发光**分析试剂盒描述:
英文名称 | Human MHCE/HLA-E (Major Histocompatibility Complex Class I E) CLIA Kit | ||
中文名称 | 人I类主要组织相容性复合体E(MHCE/HLA-E)化学发光**分析试剂盒 | ||
货号 | KL-H1363c | 种属 | Human/人 |
规格 | 96T/Kit (8*12 wells) | ||
检测方法 | 双抗体夹心法 | ||
检测范围 | 62.5~4000pg/mL | 灵敏度 | 37.5pg/mL |
人I类主要组织相容性复合体E(MHCE/HLA-E)化学发光**分析试剂盒试剂盒组成:
中文名称 | 英文名称 | 规格 | 保存 |
CLIA酶标板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
冻干标准品 | Reference Standard | 2 支 | 4℃/-20℃ # |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
发光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
发光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Manual | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 | |
特别说明: #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
1.在各孔中加入标准品或样品各 100μL,37℃孵育90分钟
2. 倒去孔内液体,拍干,加入 100μL 生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 发光底物混合液, 37℃孵育 5 分钟左右
7. 立即测定各孔的化学发光值
8. 结果计算
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KL-ANGPT1-Hu 人血管生成素1(ANGPT1) 检测试剂盒 human Angiopoietin 1 (ANGPT1) ELISA Kit Homo sapiens human 9.375-600pg/mL 3.5pg/mL 96T 12 months
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KL-ACVA-Ra 大鼠激活素A(ACVA) 检测试剂盒 Rat Activin A (ACVA) ELISA Kit Rattus norvegicus Rat 31.25-2000pg/mL 11.3pg/mL 96T 12 months
KL-BDNF-Ra 大鼠脑源性神经营养因子(BDNF) 检测试剂盒 Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.059ng/mL 96T 12 months
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KL-bTC-Hu 人β细胞素(βTC) 检测试剂盒 human Betacellulin (bTC) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.064ng/mL 96T 12 months
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